[Article in Chinese]
           Jou R, Kan S, Yang WJ, Huang C, Chang MK, Liau MY
           Division of Biologics' Product Production, National Institute of
           Preventive Medicine, Taipei, Taiwan, R.O.C.
           Chung Hua Min Kuo Wei Sheng Wu Chi Mien I Hsueh Tsa Chih 1996
           Feb;29(1):57-64

        In order to prolong shelf-life and improve the quality of the vaccine
        product, not only an effective stabilizer but also a more proper dosage
        form has been sought. The stability of a Japanese encephalitis (JE)
        vaccine produced from mouse brain along with a variety of stabilizers
        and lyophilization protocols was evaluated. Without any stabilizers
        added, almost 90% of the antigenicity would vanish after freeze-drying
        process. Comparative studies of various compounds, including
        carbohydrates, amino acids, peptides and medium 199, on both
        antigenicity preservation and thermostability of the vaccine were
        carried out. The results indicated that the best reconstituted vaccines
        were prepared with two stabilizer formulations, sucrose and
        sucrose/gelatin. They were further examined by accelerated stability
        test at room and higher temperatures. The sucrose-added lyophilized
        vaccine can retain its original antigenicity for more than 60 days both
        at 37 degrees C and 45 degrees C. We conclude the thermostability
        efficiency of each of the stabilizers tested is as that follows: sucrose
        > sucrose/gelatin > gelatin/medium > gelatin.